Xenbase Image ID: 75620

Fig. 1. Xdazl protein is expressed in the germ plasm of Xenopus embryos. (A) Vegetal view of albino 4 cell embryos lacking Xdazl immunoreactivity. (B) Bisected (upper) and intact (lower) albino stage 10.5 embryos stained with purified antibodies to Xdazl. Arrows indicate Xdazl-stained PGCs below the blastocoel (bl, upper embryo) and in the yolk plug (lower embryo) (C) Bisected (upper) and intact (lower) albino stage 10.5 embryos stained with antiserum depleted of Xdazl antibodies. (D). Bisected (left) and intact (right) stage 13 embryos stained for Xdazl and cleared in Murray. Arrows indicate stained PGCs in the endoderm below the archenteron (ar). Anterior is toward the left. (E) Bisected stage 18 cleared embryo showing Xdazl-stained PGCs (arrows). Anterior is to the left. (F) Stage 7 section immunostained for Xdazl showing reactivity in the germ plasm (gp). The vegetal pole (vp) is toward the bottom. (G) Section through a stage 10.5 embryo showing perinuclear Xdazl staining (arrow), nucleus, n. (H) Control section from a stage 10.5 embryo incubated with antiserum depleted of Xdazl antibodies showing unlabelled germ plasm. (I) The same section in H stained with Heindenhain Azan confirming the presence of germ plasm (arrow, dark blue) around a nucleus (n, pale blue). (J) Stage 13 section with Xdazl-stained germ plasm. Scale bar, 50 μm. Image published in: Houston DW and King ML (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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