Xenbase Image ID: 75621

Fig. 4. PGCs are reduced in number or absent in Xdazl-depleted early tadpoles (stage 39/40). (A) RT-PCR analysis of Xpat RNA levels in individual uninjected (Un), oligo injected (Oligo) or rescued (Oligo+Xdazl) stage 39 embryos. EF1-alpha was assayed as a loading control. (B-F) Whole-mount in situ hybridization for Xpat RNA in uninjected (B, D) and Xdazl-depleted (C,E,F) stage 39/40 embryos. (B) Uninjected control embryos showing numerous Xpat- labelled PGCs in the posterior dorsal endoderm (arrowheads). (C) Xdazl-depleted embryos showing examples of reduced (arrowhead) or absent Xpat-labelled PGCs. (D) Close up view of PGCs in an uninjected tadpole. (E) Close up of an oligo-injected tadpole showing only four PGCs. (F) Example of an oligo-injected tadpole with no PGCs. Image published in: Houston DW and King ML (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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