Xenbase Image ID: 75623

Fig. 6. Germ plasm aggregation and perinuclear positioning occurs normally in Xdazl-depleted embryos. Uninjected (A,C,E) or Xdazl- depleted embryos (B,D,F) were fixed at stage 10, sectioned and stained with Heindenhain Azan (A-D), or were fixed at stage 12 for Xpat whole-mount in situ hybridization and subsequently sectioned (E,F). (A) Section of an uninjected stage 10 embryo showing PGCs (arrowhead) in the vegetal endoderm. (B) Section of an Xdazl- depleted stage 10 embryo with a PGC in the vegetal endoderm (arrowhead). (C) High magnification view of the germ plasm (gp) from one of the PGCs in A. (D) High magnification view of the PGC in (B) demonstrating perinuclear germ plasm accumulation in Xdazl- depleted embryos. (E) Xpat staining of germ plasm in an uninjected stage 12 embryo. (F) Xpat staining in the germ plasm of an Xdazl- depleted stage 12 embryo showing two adjacent PGCs. (G) RT-PCR analysis of Xpat expression in individual stage 12 and 26 embryos showing equal RNA abundance in uninjected and oligo-injected samples. Scale bars, 625 μm (A,B), 80 μm (C,D), 50 μm (E,F). Image published in: Houston DW and King ML (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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