Xenbase Image ID: 75819

Figure 3. Ectopic expression of PIAS genes in early frog embryos disrupts Xenopus development. A: In vitro translation showed that all PIAS proteins were synthesized efficiently from their cognate RNAs. When RNAs were injected at high doses (2 ng), PIAS1, 2, and 4 induced severe lesions in embryos at early gastrula stages and embryonic lethality before tailbud stages. PIAS3 was less effective in inducing the lesions. B: When the doses of the RNAs were adjusted to allow embryo survival (shown here were embryos injected with RNAs of 1 ng PIAS1, 0.5 ng PIAS2, 2 ng PIAS3, and 1 ng PIAS4), the resulting tailbud embryos showed failure in blastopore closure, shortened body axis, and defective head formation. The embryos with ectopic PIAS1 often displayed enlarged cement gland (arrows), and the embryos with elevated PIAS4 sometimes formed pigmented aggregates at the flank (arrowhead).Download figure to PowerPoint Image published in: Burn B et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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