Xenbase Image ID: 75919

Fig. 6. XSufu inhibits Hh/Gli signaling in the Xenopus embryo. (A) Molecular analysis by RT-PCR of animal cap (AC) explants cultured until stage 16. Animal injection of 500 pg XBhh mRNA elevates the expression of XPtc2, XPtc1, and XGli1, and this effect is reverted by co-injection of 16 ng XSufu mRNA. XSufu-MO, but not control-MO, increases the expression of these Hh target genes. H4 was used for normalization. (B,B′,C,C′) Whole-mount in situ hybridization of early neurula embryos in dorsal view. Animal injection of 15 ng XSufu mRNA reduces, while XSufu-MO, but not control-MO, expands the expression of XPtc1 expression. Indicated effects were observed in: B, 61/61; B′, 29/38; C, 58/58; C′, 32/37. Image published in: Min TH et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue ptch1.S patched, patched1, Ptc-1, Ptc1, X-ptc1, XPtc1 X. laevis Throughout NF stage 15 neural plate neural groove neuroectoderm Image source: Published Larger Image Printer Friendly View

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