XB-IMG-75920
Xenbase Image ID: 75920
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Fig. 7. XSufu inhibits Wnt/β-catenin signaling in the Xenopus embryo. (A) Molecular analysis by RT-PCR of animal cap (AC) explants cultured until stage 10. Embryos were animally injected with 25 pg XWnt8 mRNA either alone or in combination with the indicated amount of XSufu mRNA. H4 was used for normalization. XSufu mRNA downregulates XWnt8-induced transcription of Siamois and Xnr3 in a dose-dependent manner. (B) XSufu-MO, but not control-MO, induced expression of the Wnt target genes Siamois, Xnr3, and Chordin in injected AC explants at stage 10. (C–F,C′–F′) Whole-mount in situ hybridization of early gastrula embryos in dorsal view. A single marginal injection of XSufu mRNA at the 4-cell stage reduces, while a single animal injection of XSufu-MO, but not of control-MO, at the 2-cell stage expands endogenous expression of Xnr3 and Chordin. (G–G′) Animal view of early gastrulae. A single animal injection of 12 pg XWnt8 mRNA at the 4-cell stage induces ectopic Xnr3 expression (G′) that is reduced by co-injection of XWnt8 and XSufu mRNA (G′). Injected nlacZ-positive cells are highlighted with a strippled line. (H) Dorsal view of uninjected neurula embryo. Condensed natural pigmentation at the site of neural tube closure delineates the main body axis. (I–M) A single ventral injection of 4 pg XWnt8 mRNA induces a complete secondary body axis (arrowhead in I) that is blocked by co-injection with 4 ng XSufu mRNA (J) or by co-injection with a combination of XSufu and 150 pg XGli1 mRNA (K). Note that XWnt8-induced secondary axis formation is partly reversed by co-injection of 150 pg XGli1 mRNA (arrowhead in L) and inhibited by co-injection of 450 pg XGli1 mRNA (M). (N) Quantification of secondary axis formation. Indicated effects were observed in: C, 14/14; C′, 8/10; D, 15/18; D′, 24/36; E, 13/13; E′, 7/8; F, 24/26; F′, 21/28; G, 47/47; G′, 14/14; G′, 55/55. Image published in: Min TH et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
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