Xenbase Image ID: 75952

Figure 3 ADAM13 controls CNC induction through regulation of EfnB and Wnt signaling (A, B) Embryos were injected as in Figure 1E with transcripts as follows: 200 pg EfnB1 and 100 pg ADAM13 in (A), and 200 pg each in (B) along with 3 ng of control (CT) or 13-3 MO. In situ hybridization was processed for snail2 at stage 12.5/13; injected side is denoted with a red asterisk. (C) Embryos were injected in one ventral-vegetal blastomere at 16-cell stage with the indicated RNA (1.5 pg Wnt8; 100 or 200 pg EfnB1) and MO 13-3 (1.5 ng), and scored for secondary axis formation at tailbud stages. (D) HEK293T cells were transfected with pTopflash or pFopflash, pCMV-β-gal and the indicated constructs, and cultured for ~40 hrs. Cell lysates were processed for luciferase and β-galactosidase assays as controls for transfection efficiency. A representative experiment performed in triplicate is shown here. Results are presented as ratios of TOPFLASH vs. FOPFLASH luciferase activity (both were normalized for β-gal activity), and the value calculated for cells transfected with control plasmids only was set to 1. Error bars represent standard deviations. **: P < 0.001; NS: not signicicant (P = 0.71). U, uninjected. Image published in: Wei S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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