Xenbase Image ID: 75961

Figure 3. Overexpression of ADAM 13 does not affect central nervous-system formation. Whole-mount in situ hybridization of embryos expressing (a,c,d) wild-type ADAM 13 or (b,e) the E/A mutant constructs were processed with (a) the pan-neural marker Xsox-2 and (d,e) the neuronal marker N-tubulin. Black dots indicate the transcript-injected half of each embryo. Embryos expressing wild-type ADAM 13 lack Xsox-2-positive cells in the vicinity of the otic vesicle; (a) such cells are present in the E/A and noninjected control halves of each embryo (arrowheads). (c) Transverse section at the level of the putative otic vesicle of an embryo in a stage similar to that in (a). Dorsal views of both (d) wild-type and (e) E/A-expressing embryos at the early-neurula stage (stage 15). (d,e) Lateral expression of N-tubulin within the putative trigeminal ganglion (black arrowheads) is absent from (d) the wild-type-injected side. (f) Dorsal views of neurula-stage (stage 19) embryos overexpressing (f,g) wild-type or (h,i) E/A ADAM 13 and hybridized with the Xkrox-20 probe. The localization of Xkrox-20 in rhombomeres 3 and 5 is identical in the uninjected control side compared to the injected side of each embryo Image published in: Alfandari D et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue adam13.L x-adam 13 X. laevis Throughout NF stage 20 neural crest eye primordium neural tube anterior neural tube neural plate chordal neural plate pre-chordal neural plate egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 19 to NF stage 20 rhombomere R3 rhombomere R5 neuroectoderm tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Sometime during NF stage 19 to NF stage 20 neural groove neural plate trigeminal placode neural plate border chordal neural plate border Image source: Published Larger Image Printer Friendly View

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