XB-IMG-75962
Xenbase Image ID: 75962
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Figure 4. CNC grafts. Transcripts encoding GFP (0.3 ng) alone or in combination with wild-type (0.7 ng) or E/A (0.7 ng) ADAM 13 were injected in one blastomere of two-cell-stage embryos. Labeled cranial neural-crest explants were taken from stage 17/18 Xenopus embryos and grafted into unlabeled sibling embryos at the same position (CNC was removed from the host at this position). Lateral views of tailbud stage (stage 24) grafted embryos, with (a) bright field images on the left and (a′′) fluorescent images on the right. (a,a′) Embryos that were grafted with cranial-neural crest that expressed both GFP and wild-type ADAM 13 were indistinguishable from (not shown) embryos grafted with CNC expressing GFP alone. (a′) Fluorescent neural-crest cells migrate along pathways normally used by CNC streams (m, mandibular; h, hyoid; ba, branchial anterior; bp, branchial posterior). (b) In contrast, cranial neural-crest cells expressing both the E/A ADAM 13 mutant and GFP (b′) do not migrate or (c′) only migrate in the mandibular segment. The cement gland (dotted line) and (e) the future eye are used in each embryo as reference points for the evaluation of neural crest-cell position and the extent of migration Image published in: Alfandari D et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |