Xenbase Image ID: 75964

Figure 6. Xenopus XTC cells transfected with wild-type ADAM 13 remodel a fibronectin substrate. Xenopus XTC cells were plated on fluorescent fibronectin for 14 hr prior to fixation. Cells were permeabilized and processed for immunofluorescence with a mouse polyclonal antibody directed against (a) endogenous Xenopus ADAM 13 or (b) mAb 9E10 directed against the Myc-tag epitope. Fluorescence was observed with a confocal microscope. (a′′) Fluorescent fibronectin substrates correspond to the same fields as (a) immunostained cells. (a) Endogenous ADAM 13 is preferentially localized to the perinuclear endoplasmic reticulum and to cellular extensions at the cell periphery in nontransfected XTC cells (arrowheads). (a′) These cellular extensions colocalize with minor alterations in the fluorescent fibronectin substrate (arrowheads). (b) Cells transfected with wild-type ADAM 13 dramatically modified (b′) the substrate. In contrast, (c′) the fluorescent fibronectin substrate in contact with (c) E/A-transfected cells is comparable to the substrate of (a′) nontransfected control cells Image published in: Alfandari D et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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