Xenbase Image ID: 75966

FIG. 6. Localization of X-ADAM 13 mRNA in early embryos. (A) Lateral view of a blastula (stage 8) and a neurula (stage 13) stage embryo. X-ADAM 13 mRNA is not detected in the blastula. During neurulation the mRNA is localized to the anterior placodal ectoderm (arrows and arrowhead). (B) Dorsal view of a stage 13 neurula. The neural plate (np) and notochord (c) are indicated. (C) Anterodorsal view of stage 20 embryo. Three distinct groups of cells are stained in the anterior region. These populations of cells arise from the border of the neural tube and expand on either side ventrally (arrows and arrowhead). The most anterior of these groups of cells surrounds the optic vesicle (arrowhead). (D) Dorsal view of stage 20 embryo. X-ADAM 13 mRNA is also localized to the forming somites (blue arrows). At this angle, the anterior neural crest staining is observed around both the optic vesicle and the brain (arrowhead). Black arrows point to the same cells as in C. (E) During tailbud formation, the mRNA is present in the head, somites, and proctodeum (pd). The staining present in the anterior part of the embryo progresses ventrally as development proceeds (arrows). Note the extensive staining of facial structures of the head (arrowhead) with the exception of the cement gland (cg). (F) Magnification of a dorsal view of an early neurula (stage 13). When the color reaction is allowed to proceed overnight, secondary sites of specific expression become apparent. Note the paraxial mesodermotochord boundary where presumptive somite cells are detected with the antisense probe (white arrowhead). No staining is detected in the notochord (c). (G) Magnification of the dorsoanterior view of an early tailbud (stage 20). In the somites, the staining is restricted to the region containing the nuclei (white arrows) and absent from the intersomitic border (red arrowheads). Image published in: Alfandari D et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue adam13.L x-adam 13 X. laevis Throughout NF stage 20 neural crest somite periocular region cranial neural crest mandibular crest hyoid crest branchial crest adam13.L x-adam 13 X. laevis Throughout NF stage 13 preplacodal ectoderm adam13.L x-adam 13 X. laevis Throughout NF stage 25 to NF stage 27 somite visceral pouch head region proctodeum cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest Image source: Published Larger Image Printer Friendly View

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