Xenbase Image ID: 76023

Figure 1. Live imaging of fibrillogenesis. A: Epithelia-free explants are microdissected from either the animal cap (e, ectoderm) or the marginal zone (n, notochord; s, somite) of gastrulating embryos (d, dorsal anterior midline; bc, blastocoel) and cultured between two sheets of precast agarose (B). C: To collect high resolution confocal time-lapses, the tissue is gently compressed under a glass coverslip fragment (g), sandwiched between two sheets of agarose (the lower sheet as thin as 10 microns), glued in place with silicone grease (s). D,E: A sequence of frames from a confocal time-lapse of fibronectin-rich fibrils (red; labeled by incubation with a nonfunction blocking monoclonal antibody to Xenopus laevis fibronectin, 4H2, conjugated to the fluorophore Cy3) on the ventral surface of the explant (D) and a sequence of frames from the same time-lapses showing a scatter-labeled group of cells expressing RNA encoding a plasma membrane localizing GFP (E; green; GAP-43 GFP). Small fibronectin-rich spines can be seen forming perpendicular to thick fibrils (arrowheads).Download figure to PowerPoint Image published in: Davidson LA et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

Return to previous page