Xenbase Image ID: 76026

Figure 4. Disrupting cortical actin or cell adhesion rapidly alters fibril topology. A: Frames from a representative time-lapse sequence show cell plasma membrane and fibronectin-rich fibrils on the surface of animal cap ectoderm after addition of 4 μM Cytochalasin D. Within 10 min the complex network is reduced to a few thick cables. These cables remain on the ventral surface of the explant even after the cells have rounded up at 20 min. B: Frames from a time-lapse sequence where 1 mM ethylenediaminetetraacetic acid (EDTA) is added. EDTA also reduces network complexity but remaining cables penetrate deep into the tissue where the fibrils are held between cells (arrows in panel on far right in B).Download figure to PowerPoint Image published in: Davidson LA et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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