Xenbase Image ID: 76029

Fig. 2. ADAM13 metalloproteinase activity is required for eye field formation and eye morphology. One dorsal-animal blastomere of 8-cell stage embryos was injected with the indicated MO (1.5 ng) and, as indicated, rescue mRNA encoding wild-type or the E/A mutant of ADAM13 (25 pg). (A) Embryos were allowed to develop to stage ~12.5 and then processed by in situ hybridization for pax6. A representative embryo of each injection is shown in the upper panels (the injected side is denoted with a red asterisk), and combined results summarized in graphs. (B) Embryos were scored for eye phenotype at stage ~35. **, pb0.001; NS, not significant (p=0.28). See Fig. 1A and Materials and methods for phenotype scoring. Image published in: Wei S et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 12.5 pre-chordal neural plate Image source: Published Larger Image Printer Friendly View

Return to previous page