Xenbase Image ID: 76080

Fig. 2. Rfx2 is required for neural tube closure and proper neural cilia formation. (A–D) Stage 19 embryos, dorsal view, anterior top. Black dashed lines outline the distance between the neural folds. (A) Stage 19 control embryo. The neural tube is almost closed. (B) Embryo injected with Rfx2 morpholino dorsally. The neural tube closure defects are shown. The neural tube closure defects caused by disruption of Rfx2 can be partially rescued by co-injection with 150 pg GFP-Rfx2 mRNA (C) and 300 pg GFP-RFX2 mRNA (D). The average distance between the neural folds is shown in (E). (F) Transverse section view of neural plate of stage 18 control embryos. Cilia are stained with acetylated α-tubulin. (G) Transverse section view of neural plate of Rfx2 morphants. Shorter cilia are observed. (H) Cilia were measured in 15 μm projection confocal images. The average length of control cilia is 1.19±0.021 μm (mean±SEM, n=225). However, the average length significantly reduces to 0.79±0.016 μm in Rfx2 morphants (mean±SEM, n=195). Horizontal lines indicate the mean, vertical lines SEM, *** pb0.0001 Mann–Whitney test. Image published in: Chung MI et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba8.S tubal2 X. laevis Throughout NF stage 18 neural plate Image source: Published Larger Image Printer Friendly View

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