Xenbase Image ID: 76082

Fig. 4. Rfx2 is required for GRP cilia assembly and the left–right asymmetry pattern. (A) GRP tissue of a membrane RFP-injected control embryo. Acetylated bα-tubulin labels cilia (green) and RFP labeled cell boundary (red). (B) GRP tissue of a Rfx2 morphant. Membrane-RFP is co-injected as a tracer. (C) GRP tissue of an embryo injected with Rfx2 morpholino and GFP-Rfx2 mRNA. (A′), (B′), (C′) are zoom in view of (A), (B), (C), respectively. (D) Average length of GRP cilia. While the average length of control GRP cilia is 8.59± 0.18 μm (mean±SEM, n=69), it's significantly reduced to 2.34±0.07 μm in Rfx2 morphants (mean±SEM, n=113). The shorter cilia phenotype is partially rescued to 4.74± 0.14 μm(mean±SEM, n=63) by co-injecting with GFP-Rfx2 mRNA. Horizontal lines indicate the mean, vertical lines SEM, *** pb0.0001 Mann–Whitney test. (E–F, I) Pitx2c expression at stage 26, lateral view. In control embryos, Pitx2c is expressed in the left LPM (black arrow) but not the right LPM (blue arrow). (G–H) In Rfx2 morphants, bilateral LPM of Pitx2c expression is observed. (blue arrow). (I) Quantification of Pitx2c expression patterns in control embryos and Rfx2 morphants. Image published in: Chung MI et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba8.S tubal2 X. laevis Throughout NF stage 26 gastrocoel roof plate pitx2.S pitx2-A, pitx2-a, pitx2-b, pitx2c, xpitx2, xPtx2 X. laevis Throughout NF stage 26 lateral plate mesoderm cement gland primordium profundus ganglion heart primordium trigeminal ganglion Image source: Published Larger Image Printer Friendly View

Return to previous page