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Fig. 5. localization of XFD gene transcripts in Xenopus embryos by whole-mount in situ hybridization. XFD-1: embryos at gastrula (stage 11) [A,B] and transverse section through an embryo at early neurula (srage 13) [C]. XFD-2: blastula (stage 8) embryo [D], section through the animal cap [E] and late gastrula (stage 12) embryo [F]. XFD-3: transverse section through an embryo (stage 23) at the anterior hindbrain region [G]. stage 23 [H] and tai1bud (stage 28) (I) embryos. XFD-4: embryos at stage 16 [J]. stage 32 [K] and stage 37 [L]. Arrows [J] denote pre-somitogenic mesoderm, arrowhead [K] points towards intense staining at the tip of the tail. XFD-6: embryos at stage 17 [M]. stage 19 [N] and stage 23 [O]. Arrows and arrow. heads denote two types of cell populations differentiating to cranial neural crest cells of the hindbrain (arrows) and midbrain (arrowhead). XFD-7: neurula (stage 15) (PI, stage 35 (0) and stage 44 (R) embryos. Arrowhead (R) points at localized transcription in a defined area of the gut at the right side of the embryo XFD-9: Embryos at stage 16 (S), stage 25 (T). stage 35 [U] and transverse section through a stage 36 embryo [V] at the middle part. Arrowheads in (T) denote the border between segmented and unsegmented somitic mesoderm. arrowheads in (U) point at intense staining near the posterior pole XFD-l0: Neurula (stage 15) embryo [W]. lateral view of a neurula Istage 16) embryo [X] and dorsal view at an embryo at stage 24/25 (Y). Arrowheads in (V) point at a cranial neural crest cells. b, blastocoel; bp, blastoporus; d and v, dorsal and ventral lip of blastoporus; f, neural floor plate; g, foregut; h, heart; n, notochord; p, pronephros.
