Xenbase Image ID: 76174

Fig. 6. Spatial expression profile of enpp1, 4, 6 and 7 during development. Whole mount in situ hybridisation with DIG-labelled antisense RNA probes was performed on embryos from stages 5-41 for enpp1, 4, 6 and stages 32-45 for enpp7. All embryos were analysed from the same experimental set. The differences in colours result from clearing the embryos and photographing them against a white background to visualise staining. (A) Whole-mount in situ hybridisation analysis of enpp1 expression. Lateral view at stages 28 (i), 35/36 (ii) and 37/38 (iii). Detail of the fin at stage 41 (iv) and dorsal view of a stage 41 embryo (v). Anterior is right and dorsal is up, except in (v). da, dorsal aorta; dc, duct of cuvier; dlav, dorsal longitudinal anastomosing vessel; h, heart; pcv, posterior cardinal vein; ps, pronephric sinus; vbi, ventral blood island. (B) Whole-mount in situ hybridisation analysis of enpp4 expression. Animal view at stage 5 (i), anterior view at stage 22 (ii), lateral view at stages 32 (iii), 37/38 (iv) and 41 (v). For the lateral views, dorsal is up and anterior is right. Transverse sections at stage 37/38 through the proximal tubules (vi). Dorsal is up. The dotted line through the pronephric tubules (iii) correspond to planes of section in (vi). cg: cement gland; ct, collecting tubules; dt, distal tubules; hg, hatching gland; pt, proximal tubules; s, somites. (C) Whole-mount in situ hybridisation analysis of enpp6 expression. Lateral view at stage 5 (i), lateral view at stage 28 (ii) and 37/ 38 (iii). Dorsal is up and anterior is right. Transverse sections in the proximal tubules level at 37/38 (iv). The dotted line through the pronephric tubules correspond to planes of section in (iv). p, pronephros; pt, proximal tubules. (D) Whole-mount in situ hybridisation analysis of enpp7 expression. Ventral view of stage 45 embryos hybridized with anti-sense (i) and sense (ii) probe, lateral view of the gut region of a stage 45 embryo (iii), cryostat section (iv) at 28μm of a stage 45 embryo through the gut. no, notochord; m, muscles; sc, spinal cord; sia, first sections of the small intestine; sib, external coil of small intestine; sic, internal coil of small intestine (from Chalmers and Slack, 1998). Image published in: Massé K et al. (2010) Copyright © 2010. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue enpp6.L X. laevis Throughout NF stage 5 (16-cell) animal hemisphere animal pole enpp6.L X. laevis Throughout NF stage 37 and 38 late proximal tubule early proximal tubule pronephric tubule enpp6.L X. laevis Throughout NF stage 28 hindbrain pronephric mesenchyme foregut otic vesicle rhombomere enpp4.L X. laevis Throughout NF stage 5 (16-cell) animal hemisphere animal blastomere enpp4.L X. laevis Throughout NF stage 22 hatching gland cement gland primordium enpp4.L X. laevis Throughout NF stage 32 brain somite pronephric tubule cement gland forebrain otic vesicle distal tubule proximal tubule enpp4.L X. laevis Throughout NF stage 37 and 38 cloaca somite early proximal tubule late proximal tubule cement gland spinal cord notochord enpp4.L X. laevis Throughout NF stage 41 pronephric duct hindgut somite pronephric tubule early proximal tubule spinal cord axial mesoderm mouth primordium enpp1.L X. laevis Throughout NF stage 28 notochord ventral blood island otic vesicle enpp1.L X. laevis Throughout NF stage 35 and 36 notochord forebrain otic vesicle lateral line placode supraorbital lateral line ventral blood island trigeminal ganglion enpp1.L X. laevis Throughout NF stage 37 and 38 heart posterior cardinal vein ventral blood island dorsal aorta head region head vasculature dorsal longitudinal anastomosing vessel duct of Cuvier internal carotid artery enpp1.L X. laevis Throughout NF stage 41 dorsal longitudinal anastomosing vessel pronephric sinus posterior cardinal vein dorsal aorta head vasculature brain enpp7.L X. laevis Throughout NF stage 45 small intestine external coil of the small intestine Image source: Published Larger Image Printer Friendly View

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