XB-IMG-76184
Xenbase Image ID: 76184
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Fig. 1. In situ hybridizations during embryonic (A,B), larval (C), metamorphic (E,F) and post-metamorphic stages (D). (A,B) Whole-mount col2a1 in situ hybridization of a Nieuwkoop- Faber (NF) stage-33/34 embryo. (A) Lateral view, anterior is to the right. There is strong expression of col2a1 in the notochord (NO), otic vesicle (OV) and branchial arches (BA). Black line points to level of section depicted in (B). (B) Histological cross section through the trunk. Note diffuse staining in the somites (SO) and floor plate of the neural tube (FP). (C) Whole-mount col2a1 in situ hybridization; stage-46 tadpole head. Ventral view, anterior is to the top. There is strong expression in early larval cartilages, including the ceratobranchials (CB), but not in the interhyoideus muscle (IH). (D) Sectioned in situ hybridization of a stage-64 froglet nasal capsule. Coronal section, anterior is to the top. Col2a1 is expressed again in the adult cranial cartilages, including the post-metamorphic nasal septum (NS), planum terminale (PT) planum antorbitale (PA), and alary cartilage (AC). (E,F) Mid- metamorphic, stage-57 tadpole. Coronal histological sections of the nasal capsule, stained by in situ hybridizations with col2a1 and sox9 probes. (E) Col2a1 expression increases in the alary (AC) and superior prenasal (SPC) cartilages as they form during metamor- phosis. (F) Up-regulation of col2a1 corresponds with heightened expression of sox9 in these cartilages during metamorphic chon- drogenesis. Additional abbreviations: NC, nasal capsule; EY, eye. Scale bar, 0.5 mm. Image published in: Kerney R et al. (2010) Copyright © 2010. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View |