Xenbase Image ID: 76186

Fig. 4. Chondrocyte expression of reporter constructs in histological sections. (A,B) Tadpole injected with a reporter construct that contains intronic region B. Coronal sections through a single larval ceratobranchial cartilage stained with anti-collagen II antibody (red in A) or anti-GFP antibody (green in B) with DAPI nuclear counter stain (blue). (C,E,G) Trichrome and (D,F,H) merged bright-field and fluorescent images of anti-GFP antibody stained sections. (C,D) Coronal sections of the nasal capsule of a post-metamorphic froglet that carries the reporter construct with intronic region B. (D) EGFP is expressed in the alary cartilage (AC), nasal septum (NS) and planum terminale (PT). (E,F) Longitudinal sections through the leg of a froglet that carries a reporter construct with intronic region B. Cartilaginous expression is seen in the humerus (HU) and proliferating chondrocytes (PC) of the radio-ulna (RU). EGFP is not expressed in the hypertrophic (HC) or articular (AR) chondrocytes of the radio-ulna. (G,H) Serial coronal sections through the ethmoidal region of a froglet that carries the C.1 reporter. Cartilaginous expression is seen in the same post-metamorphic cartilages shown in D. Scale bar, 0.25 mm. Image published in: Kerney R et al. (2010) Copyright © 2010. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

Return to previous page