Xenbase Image ID: 76213

Fig. 2. Tbx6 is required for bowline and ledgerline expression during Xenopus development. (A) The ex- pression of bowline and ledgerline is induced by the synergistic effects of Tbx6, mespb, and E47. RT-PCR analysis shows that overexpression of the mixture of Tbx6, mespb, and E47 mRNA species induces ectopic bowline and ledgerline expression in gastrula-stage Xeno- pus embryos. ODC (ornithine decarboxylase) was used as an internal control. (B-E) Expression of bowline mRNA and ledgerline mRNA in Xtbx6 exint-MO-injected embryos. All the embryos were fixed at stage 19 for whole-mount in situ hybridization to detect the expression of bowline (B,C) and ledgerline (D,E). Magenta-Gal staining indicates the injected side. (B,D) A representative embryo injected with 50 ng Xtbx6 exint-MO. Inhibition of the splicing of Tbx6 mRNA results in loss of expression of bowline and ledgerline. The white arrowheads in (B) and (D) indicate reduced bowline and ledgerline expression, respectively. (C,E) Representative embryos injected with 50 ng Xtbx6 exint-MO and 2 pg or 10 pg Xenopus Tbx6 mRNA, respectively. The white arrowheads in (C,E) indicate rescued bowline and ledgerline expression, respectively. Image published in: Hitachi K et al. (2009) Copyright © 2009. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue ripply2.2.S bowline, LOC100487498, ripply2.2-a, ripply2.2-b X. laevis Throughout NF stage 19 somite ripply2.1.S ledgerline, stripy X. laevis Throughout NF stage 19 somite Image source: Published Larger Image Printer Friendly View

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