Xenbase Image ID: 76217

Fig. 7. xRipply3 suppresses the transcriptional ac- tivity of Tbx1. (A) Schematic diagram of pGL4-Tbox, which contains tandem T-box binding sites. (B,C) Luciferase assays for the pGL4-Tbox construct. (B) Tbx1 dose-dependently increases the luciferase activ- ity of pGL4-Tbox. COS7 cells were transfected with the pGL4-Tbox (100 ng) and the Tbx1 expression vector (50-200 ng). (C) xRipply3 represses the tran- scriptional activity of Tbx1 in the pGL4-Tbox construct. COS7 cells were transfected with pGL4-Tbox (100 ng) and a combination of the indicated expression vectors, Tbx1 (50 ng) and xRipply3 (25-100 ng). Error bars represent the SEM of three independent experiments. (D) Interaction between the xRipply3 and Tbx1 proteins in Xenopus embryos. Protein extracts of Xenopus embryos co-injected with myc-Tbx1 mRNA and HA-xRipply3 mRNA (500 pg each) were subjected to co-immunoprecipitation with the anti-myc antibody. (E) Interaction between the xRipply3 and Grg4 proteins in Xenopus embryos. Protein extracts of Xenopus embryos co-injected with myc-Grg4 mRNA and HA-xRipply3 mRNA (500 pg each) were subjected to co-immunoprecipitation with the anti-myc antibody. The asterisks indicate non-specific bands. (F-H) Frontal sections of Xenopus embryos were stained for xRipply3 (F), Fgf8 (G), and Tbx1 (H) transcripts. The regions of xRipply3 and Fgf8 expression differ from those of Tbx1. Image published in: Hitachi K et al. (2009) Copyright © 2009. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue ripply3.S LOC100145593, Xdscr6, XRipply3 X. laevis Throughout NF stage 33 and 34   fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 33 and 34   tbx1.S cafs, cthm, dgcr, dorv, tbx1-a, tbx1-b, tbx1c, vcfs, xtbx1 X. laevis Throughout NF stage 33 and 34   Image source: Published Larger Image Printer Friendly View

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