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Fig. 4. Expression of neural markers in embryos lacking REEP4 function. X. laevis or X. tropicalis embryos were injected, respectively, with 90ng MO or 30ng MO2. Control embryos received the same amounts of control MO. They were cultured to neurula (stage 15-18) or tailbud (stage 29/30) stages and analysed by in situ hybridisation for expression of N-tubulin, Sox3, Pax6 and Pax3. Expression of the neural markers N-tubulin, Sox3 and Pax6 is normal in embryos of X. laevis at neurula stages (A,B; E,F; I,J) and has declined little by tailbud stages (C,D; G,H; K,L). Early expression of the neural crest marker Pax3 is little affected by loss of REEP4 function (M,N), but expression in somites and pronephros becomes disrupted at tailbud stages (O,P). More prolonged culture to stage 38 (Q-T) reveals that Islet1 expression becomes disrupted in embryos lacking REEP4. Thus, in embryos of X. laevis, some embryos injected with a REEP4 MO appear normal (R') but in others, Islet1 expression is reduced. This phenotype is observed more frequently in embryos of X. tropicalis (T') Image published in: Argasinska J et al. (2009) Copyright © 2009. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 18 neuron Rohon-Beard neuron tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 26 forebrain pineal gland cranial placode trigeminal placode spinal cord sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 18 neural plate sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 26 brain forebrain midbrain hindbrain spinal cord lens epithelium epibranchial placode otic placode trigeminal nerve pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 18 anterior neural ridge neural plate border pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 28 otic vesicle eye midbrain spinal cord pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 18 neural plate anterior neural fold pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 28 pronephric kidney hindbrain spinal cord isl1.S islet-1, islet1, Xisl-1, Xislet-1 X. laevis Throughout NF stage 28 cranial placode post-anal gut spinal cord isl1 islet-1, islet1, Xisl-1, Xislet-1 X. tropicalis Throughout NF stage 37 and 38 post-anal gut hindbrain spinal cord Image source: Published Larger Image Printer Friendly View

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