Xenbase Image ID: 76233

Fig. 5. Activation of muscle markers in embryos lacking REEP4 is normal during neurula stages but their expression becomes disorganised or reduced thereafter. X. laevis (A-BB) or X. tropicalis (CC-FF) embryos were injected, respectively, with 90 ng MO or 30 ng MO2. Control embryos received the same amounts of control MO. They were cultured to neurula or tailbud stages and analysed by in situ hybridisation for expression of Myf5 MyoD, Mrf4, the 12/101 epitope, Dystrophin, Cardiac actin and Myosin Heavy Chain. Note in X. laevis that expression of markers at the neurula stage is normal (A,B; E,F; U,V; Y,Z) but that they become disorganised (C,D; G,H; K,L; O,P; S,T) or reduced (W,X; AA,BB) thereafter. The decrease in Cardiac actin expression in the heart in (X) is not a consistent observation. Expression of the 12/101 epitope in X. tropicalis is more sensitive to loss of REEP4 function than is expression in X. laevis. Note the decreased levels of 12/101 staining in (DD) compared with (P). Image published in: Argasinska J et al. (2009) Copyright © 2009. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue myf5.L myf-5, Xmyf-5, Xmyf5 X. laevis Throughout NF stage 15 presomitic mesoderm myf5.L myf-5, Xmyf-5, Xmyf5 X. laevis Throughout NF stage 26 pharyngeal arch myoblast myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 presomitic mesoderm myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 26 somite myf6.L herculin, mrf4, myf6-a, myf6-b, Xmrf4, XMRF4-a, XMRF4a X. laevis Throughout NF stage 15   myf6.L herculin, mrf4, myf6-a, myf6-b, Xmrf4, XMRF4-a, XMRF4a X. laevis Throughout NF stage 28 somite dmd.2.S bmd, cmd3b, dystrophin X. laevis Throughout NF stage 37 and 38 somite actc1.L actc, actna1, cardiac actin, cardiac alpha actin, muscle actin X. laevis Throughout NF stage 40 myotome heart myocardium actc1.L actc, actna1, cardiac actin, cardiac alpha actin, muscle actin X. laevis Throughout NF stage 15 presomitic mesoderm myh4.L mhc, MHC A7, MHC E3, myh2b, myhc E3, myhc-2b, myhc-iib, myhe3 X. laevis Throughout NF stage 15 presomitic mesoderm myh4.L mhc, MHC A7, MHC E3, myh2b, myhc E3, myhc-2b, myhc-iib, myhe3 X. laevis Throughout NF stage 40 somite muscle Image source: Published Larger Image Printer Friendly View

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