Xenbase Image ID: 76256

Fig. 6. Overexpression of xCyp26c could attenuate retinoic acid (RA)-induced posteriorization. (A-F) Phenotype of tad- pole injected with xCyp26c mRNA. (A-C) Normal tadpoles. (D- F) Tadpoles injected with 1 ng of xCyp26c mRNA into the animal pole region. (B,C,E,F) Histological section of xCyp26c-injected tadpoles. Sections were stained by hematoxylin-eosin. (B,E) Sagittal sections, whereas (C,F) show transverse sections of (A,D), respectively. (G-I) Head defect by RA treatment was rescued by xCyp26c injection. Embryos not injected (G) or xCyp26c mRNA-injected embryos (H) were treated with 10-6 M of RA. In xCyp26c-injected embryos, loss of eye structure was rescued (arrow). These results are summarized in graph (I). White box, dotted box, and filled box show normal eye, small eye, and no eye structure, respectively. Point out that the size of eye vesicle reflects the severity of posteriorizaion by RA treatment. (J) RT-PCR analysis with cDNAs synthesized from whole embryo injected with β-gal (1 ng; lanes 1-3) or xCyp26c (1 ng; lanes 4-6). Embryos were treated with 10-7 M (lane 2, 5) or 10-6 M (lane 3, 6) of RA, or untreated (lane 1, 4). Overexpres- sion of xCyp26c attenuated the inhibition of xBF-1 (telencepha- lic marker; column 1) and xRX1 (diencephalic marker; column 2) expression by RA treatment (lane 6). (K-N) Change of expres- sion patterns of En2 and xkrox20 by xCyp26c injection. (L,N) are magnified views of (K,M), respectively. Injected side (inj. In (K) and (M)) was indicated by lineage tracer (Red-gal). (K,L) show no RA-treated embryos and (M,N) show 10-7 M RA-treated embryos. Normal embryos showed a slight posterior shift of xkrox20 expression in the xCyp26c-injected area (white arrow- head, compared with noninjected region (black arrowhead)). RA-treated embryos showed an obvious difference in xkrox20 expression patterns between injected and noninjected regions. Image published in: Tanibe M et al. (2008) Copyright © 2008. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 18 presumptive midbrain-hindbrain boundary egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 18 rhombomere R3 rhombomere R5 Image source: Published Larger Image Printer Friendly View

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