Xenbase Image ID: 76257

Fig. 1. Over-expression and repletion of TBP inhibits axis formation. (A) Both over-expression of TBP and high-dose injection of TBP-MO into the future marginal zone region of all blastomeres of the 4-cell embryo reduced expression of Xbra. (B) Control embryo (st. 30). (C) Over-expression of TBP (700 pg/blastomere) into the future marginal zone region of dorsal blastomeres of the 4-cell embryo interfered with axis formation, including a short axis and absence of most of the head. (D) Over-expression into the ventral blastomeres of the 4-cell embryo had no effect on embryogenesis. (E) High-dose injection of TBP-MO (10 ng/blastomere) into the marginal zone region of dorsal blastomeres of 4-cell embryo also interfered with axis formation. (F) High-dose injection of TBP-MO into the ventral blastomeres of the 4-cell embryo had no significant effect on embryogenesis. (G) Over- expression of TBP reduced expression of chordamesodermal genes (center lane) at gastrula stage (st. 10). High-dose injection of TBP-MO reduced expression of all marker genes except for Xnot, which was increased by injection of TBP-MO. (H) Results of a luciferase assay using the reporter gene containing the promoter of goosecoid are shown. The luciferase activity was decreased by both over-expression of TBP and injection of TBP-MO at stage 10. Image published in: Goto T et al. (2008) Copyright © 2008. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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