Xenbase Image ID: 76258

Fig. 4. The confirmation of the speci- ficity of each morpholino. TBP-MO, TBP2-MO and TLF-MO reduced spe- cifically translation of GFP containing sequences targeted with TBP-MO, TBP2-MO and TLF-MO at the 5region, respectively. GFP-TBP (250pg), GFP- TBP2 (250pg) or GFP-TLF (250pg) with Alexa594-dextran (300pg) was injected in the animal pole of 4-cell embryo. The fluorescent signals were observed at stage 10. Pictures on top panels indi- cate the injected embryos. Pictures on center panels indicate tracer signals with Alexa594-dextran. Pictures on bottom panels indicate GFP signals. Each MO reduced translation of each corresponding GFP construct, but did not reduce translation of the GFP con- struct containing different targeting sequence. Image published in: Goto T et al. (2008) Copyright © 2008. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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