Xenbase Image ID: 76262

Figure S4. Hairy2 overexpression does not induce major cell fate changes in the early embryo. (A) In neurula embryos, Hairy2-GR mRNA (500 pg) injection represses the expression of the MyT1, N-tubulin and NeuroD neuronal markers (a, 50% n = 22; b, 55% n = 18; c, 52% n = 27). No significant change is observed on the expression of neural plate (Sox3), mesodermal (MyoD), placodal (Six1, Six3, Pax8) and antero-posterior patterning markers (Otx2, HoxB9, XAG1). All unaffected: d, n = 15; e, n = 34; f, n = 14; g, n = 17; h, n = 24; i, n = 25; j, n = 16; k, n = 25; l, n = 20. Hairy2-GR mRNA (500pg) represses the epidermal marker Epi. K. (m, 64% n = 26 and expands transiently Sox2 at neurula stages (n, 66% increase n = 18) but have no effect on Sox2 at tailbud stages (o, 100% unaffected n = 19). (B) Hairy2-GR overexpression (500 pg) decreases N-tubulin (a, 75% n = 12; b, 53% n = 19; c, 55% n = 20; d, 50% n = 12) and does not affect Sox3 and Six1 expression in stage 13 to 26-27 embryos. (e-l, all unaffected; n = 20). Embryos are shown in dorso- anterior views with the injected side to the right (a-n) or in lateral view with inset showing the control side (o). β-galactosidase was used as a lineage tracer. (C) Q-PCR analysis of stage 14 NC induced animal caps derived from embryos injected with Hairy2 MO5mis (5 ng/blastomere), Hairy2 MOs (5 ng/blastomere) or Hairy2-GR mRNA (250 pg/blastomere). Note that both Hairy2 gain or loss of function reduces Slug (a) without significantly affecting Six1, Sox3 or N-tubulin expression. Ep. keratin expression which is strongly reduced in NC induced explants, appears also unaffected by Hairy2(c). Image published in: Nichane M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue myt1.S X-MyT1, xmyt1, XMyTI X. laevis Throughout NF stage 19 trigeminal placode neuron Rohon-Beard neuron chordal neural plate anterior neural fold tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 19 trigeminal placode neuron Rohon-Beard neuron neurod1.L neurod, neuroD, neurod1-a, neurod1-b, XNeuroD X. laevis Throughout NF stage 19 trigeminal placode neuron sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 19 trigeminal placode neural plate pre-chordal neural plate chordal neural plate neural tube nrp1.L A5 antigen, LOC108718763, neuropilin, neuropilin-1, np-1, npn-1, nrp, nrp-1, pNPG152 X. laevis Throughout NF stage 19 neural tube neural plate pre-chordal neural plate chordal neural plate anterior neural fold trigeminal placode myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 19 presomitic mesoderm six1.L XSix1 X. laevis Throughout NF stage 19 anterior neural fold anterior neural ridge six3.L hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 X. laevis Throughout NF stage 19 pre-chordal neural plate optic field pax8.L pax-8, XPax-8, XPax8 X. laevis Throughout NF stage 19 lateral plate mesoderm anterior dorsal lateral plate region otic region otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 19 pre-chordal neural plate anterior neural fold optic field hoxc9-like.L X. laevis Throughout NF stage 19 chordal neural plate posterior neural tube ag1 ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 Xenopus Throughout NF stage 19 cement gland primordium krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 19 epidermis non-neural ectoderm sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 19 neural plate pre-chordal neural plate chordal neural plate sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 28 forebrain telencephalon diencephalon midbrain hindbrain trigeminal ganglion cephalic nerve retina tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 13 neuron trigeminal placode tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 16 neuron Rohon-Beard neuron trigeminal placode tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 22 neuron Rohon-Beard neuron trigeminal ganglion tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 27 neuron Rohon-Beard neuron trigeminal ganglion sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 13 neural plate pre-chordal neural plate chordal neural plate trigeminal placode sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 16 neural plate pre-chordal neural plate chordal neural plate neural tube trigeminal placode sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 22 brain forebrain midbrain hindbrain neural tube posterior neural tube anterior neural tube trigeminal ganglion sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 27 brain forebrain midbrain hindbrain trigeminal ganglion six1.L XSix1 X. laevis Throughout NF stage 13 anterior neural ridge six1.L XSix1 X. laevis Throughout NF stage 16 anterior neural fold anterior neural ridge six1.L XSix1 X. laevis Throughout NF stage 22 trigeminal ganglion otic region six1.L XSix1 X. laevis Throughout NF stage 27 trigeminal ganglion otic vesicle olfactory region Image source: Published Larger Image Printer Friendly View

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