Xenbase Image ID: 76370

Supplementary Fig. 1. Down-regulation of miRNA expression in morphants. Examples of whole mount in situ hybridisation with 4 different miRNA probes on st. 33 Xenopus embryos that were unilaterally injected at 4-cell stage with Xdcr-Mo1. wt indicates control uninjected side, mo shows injected side, which was lac-z traced and detected by salmon-Gal staining (visible as a nuclear red staining). Numbers indicate the miRNA corresponding to the probe sequence. As in these examples, hybridisation signal was always lower in the injected side compared to the control side when using these and 2 additional probes (not shown), on embryos injected either with Xdcr-Mo1 or Xdcr-Mo2 in two independent experiments. Image published in: Decembrini S et al. (2008) Copyright © 2008. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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