Xenbase Image ID: 76377

Figure 3. R-Spondin 3 Is Required for Head Cartilage Morpho- genesis (A and B) Tadpole stage Xenopus embryos injected at 8-cell stage with 10 ng per embryo of Rspo3 Mo into the animal four blastomeres. Note reduced head in Rspo3 Morphants (68%, n = 98) compared to Co Mo (0.1%, n = 85). (C, D, G, and H) Ceratobranchial cartilage was stained with Alcian blue and dissected from stage 46 embryos injected at 8-cell stage animally with either 10 ng of Rspo3 Mo or 2.5 ng of Wnt5a Mo per embryo. Note compact cartilage elements. (E, F, I, and J) Ceratobranchial cartilage was dissected from Morpholino injected embryos and flattened. The length-to-width ratios (R) of chondrocytes were determined and are indicated. (E0 and F0) Schematic drawing of cell outlines of (E) and (F). Abbreviations: br, ceratobranchial cartilage; ba, basihyal cartilage. Larger Image Printer Friendly View

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