Xenbase Image ID: 76382

Figure 3. PRR Is Required for Planar Cell Polarity (A) Dorsal view of adult thoraces of the indicated genotypes. dsRNA hairpins were expressed under the control of apGal4 at 25ﰉC. RNAi of dPRR causes planar cell polarity-like phenotypes (B) that can be rescued by overexpression of hPRR (D). (E and F) Phalloidin staining of pupal wings of the indicated genotypes aged for w32 hr after pupal formation (APF), distal side to the left. Line indicates the approximate expression domain of ptcGal4. Note the affected ptc expression domain in dPRR RNAi animals. (G) Phalloidin staining of a pupal wing of the genotype ptcGal4/UAS-dPRR RNAi1 at 40 hr APF. (H) Adult wing of the genotype ptcGal4/UAS-dPRR RNAi1 at 25dC, anterior to the left. (I) Loss of PRR function causes gastrulation defects in Xenopus embryos. Top: four-cell stage embryos were microinjected equatorially into two dorsal blastomeres with PRR morpholinos (Mo). Note the stunted axis in embryos injected with PRR Mo (75%, n = 32), but not control Mo (0%, n = 30), embryos. Middle: in situ hybridization for Xnot at gastrula stage (stage 12). Bottom: PRR Mo inhibits elongation of activin-treated animal caps. (J) ATF2-luciferase reporter assay in Xenopus embryos. Four-cell stage embryos were injected equatorially with ATF2-Luc reporter plasmid, the indicated antisense morpholinos, and mRNAs. Luciferase reporter assays were carried out from whole embryos harvested at gastrula stage (stage 12). Data are shown as mean 6 standard deviation. Image published in: Buechling T et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue not flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. tropicalis Throughout NF stage 12 presumptive axial mesoderm upper blastopore lip Image source: Published Larger Image Printer Friendly View

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