Xenbase Image ID: 76393

Fig. 4. Inhibition of WNT signalling requires the CT domain of CTGF. (A) Domain structures of CTGF and CTGFδCT. (B-D) CTGFδCT cannot inhibit induction of secondary axes by Xwnt8. (B) Secondary axis induced by Xwnt8. (C) Inhibition of secondary axis formation by CTGF. (D) CTGFδCT cannot inhibit secondary axis formation. (E) CTGFδCT is a poor inhibitor of Xwnt8-induced activation of the TOPFLASH reporter; CTGF cannot inhibit activation of the TOPFLASH reporter by Dishevelled. Both blastomeres of Xenopus embryos at the 2-cell stage received injections of 20 pg TOPFLASH DNA, 10 pg pRLTK DNA and the indicated combinations of 1 ng CTGF RNA, 1 ng CTGFδCT RNA, 50 pg Xwnt8 RNA and 1 ng Dishevelled RNA. Animal caps were dissected at stage 8, and 20 caps per sample were assayed for Firefly and Renilla luciferase activities 3 hours later. Firefly luciferase activities were then normalised to Renilla activities. This experiment represents a typical result out of three independent experiments. (F-H) CTGFδCT cannot inhibit activin-induced elongation of Xenopus animal caps. (F) Animal caps treated with 8 units/ml of activin protein undergo elongation. (G) Animal caps derived from embryos injected with 1 ng Ctgf RNA do not undergo elongation. (H) Elongation of animal caps is not inhibited by 1 ng of CtgfδCT RNA. Image published in: Mercurio S et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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