XB-IMG-76434
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Fig. 2. Distribution of competence for neural crest and panplacodal induction. (A-K′) Experimental overview. After grafting mycGFP-labeled or pigmented ectoderm from various locations and stages into the lateral neural plate border (NB) of stage 12-13 hosts (A,H), expression of panplacodal (Six1), neural crest (FoxD3) or neural (Sox3) markers was analyzed in tailbud stage embryos (B-K). (B′-K′) Sections through grafts. Arrows highlight graft borders. Asterisks indicate expression in graft, whereas arrowheads indicate expression adjacent to graft. (B-D′) After grafting stage 13 neural plate (NP) into the NB, FoxD3 but not Six1 is induced and Sox3 is maintained in the graft (inset in B, control side; inset in C′, magnified view of boxed area, arrows indicate pigment granules). (E-G′) After grafting stage 13 belly ectoderm (B) into the NB, Six1 but neither FoxD3 nor Sox3 is induced in the graft. (I-K′) After grafting stage 9 animal caps (AC) into the NB, FoxD3 and Sox3 but not Six1 are induced in the graft (inset in K, Sox3 is more widely induced in grafts placed into stage 13 NB; ab in J′, air bubble). (L,M) Comparison of FoxD3, Sox3 and Six1 induction after grafting belly (L) or neural plate ectoderm (M) into NBs of different stage hosts. Stage 12 and stage 13 NB are more conducive to FoxD3 and Six1 induction, respectively. (N) Decline of FoxD3 and Sox3 induction, and increase in Six1 induction with increasing age of belly ectoderm grafted into the NB. Data for Six1 at stage 13 taken from Ahrens and Schlosser (Ahrens and Schlosser, 2005). (O) Decline of Six1 induction with increasing age of neural plate ectoderm grafted into the NB. Image published in: Pieper M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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