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Fig. 4. Effects of Dlx3 or GATA2 gain or loss of function on neural ectodermal markers. (A-G′′) Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. For Msx1, additional examples of embryos are shown in the insets in C and C′. Image published in: Pieper M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 20 neural plate pre-chordal neural plate chordal neural plate anterior neural fold anterior placodal area zic1.S odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Throughout NF stage 18 neural plate border cranial neural crest msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Throughout NF stage 18 neural plate border anterior placodal area pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 18 neural plate border pre-chordal neural plate border chordal neural plate border anterior placodal area foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 18 cranial neural crest sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 18 neural crest cranial neural crest trunk neural crest anterior placodal area tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 18 Rohon-Beard neuron neuron anterior placodal area Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.a Expression Phenotype decreased amount sox3.L expression in neuroectoderm decreased amount sox3.L expression in non-neural ectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.a' Expression Phenotype decreased amount sox3.L expression in neuroectoderm decreased amount sox3.L expression in non-neural ectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.a^1 Expression Phenotype increased amount sox3.L expression in neuroectoderm mislocalised sox3.L expression in neuroectoderm decreased amount sox3.L expression in non-neural ectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.a^3 Expression Phenotype increased amount sox3.L expression in neuroectoderm mislocalised sox3.L expression in neuroectoderm decreased amount sox3.L expression in non-neural ectoderm Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.b Expression Phenotype decreased amount zic1.S expression in neuroectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.b' Expression Phenotype decreased amount zic1.S expression in neuroectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.b^1 Expression Phenotype increased amount zic1.S expression in neuroectoderm mislocalised zic1.S expression in neuroectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.b^3 Expression Phenotype increased amount zic1.S expression in neuroectoderm mislocalised zic1.S expression in neuroectoderm Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.c Expression Phenotype decreased amount msx1.L expression in neuroectoderm decreased amount msx1.L expression in non-neural ectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.c' Expression Phenotype decreased amount msx1.L expression in non-neural ectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.c^1 Anatomical Phenotype increased width of neural plate Expression Phenotype mislocalised msx1.L expression in neuroectoderm increased amount msx1.L expression in neuroectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.c^3 Expression Phenotype mislocalised msx1.L expression in neuroectoderm increased amount msx1.L expression in neuroectoderm Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.d Expression Phenotype decreased amount pax3.S expression in neuroectoderm decreased amount pax3.S expression in non-neural ectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.d' Expression Phenotype decreased amount pax3.S expression in neuroectoderm decreased amount pax3.S expression in non-neural ectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.d^1 Anatomical Phenotype increased width of neural plate Expression Phenotype mislocalised pax3.S expression in neuroectoderm increased amount pax3.S expression in neuroectoderm decreased amount pax3.S expression in non-neural ectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.d^3 Anatomical Phenotype increased width of neural plate Expression Phenotype mislocalised pax3.S expression in neuroectoderm increased amount pax3.S expression in neuroectoderm decreased amount pax3.S expression in non-neural ectoderm Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.e Expression Phenotype decreased amount foxd3.L expression in neuroectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.e' Expression Phenotype decreased amount foxd3.L expression in neuroectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.e^1 Anatomical Phenotype increased width of neural plate Expression Phenotype decreased amount foxd3.L expression in neuroectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.e^3 Anatomical Phenotype increased width of neural plate Expression Phenotype mislocalised foxd3.L expression in neuroectoderm increased amount foxd3.L expression in neuroectoderm increased amount foxd3.L expression in non-neural ectoderm mislocalised foxd3.L expression in non-neural ectoderm Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.f Expression Phenotype decreased amount sox9.L expression in neuroectoderm decreased amount sox9.L expression in non-neural ectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.f' Expression Phenotype decreased amount sox9.L expression in neuroectoderm decreased amount sox9.L expression in non-neural ectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.f^1 Anatomical Phenotype increased width of neural plate Expression Phenotype decreased amount sox9.L expression in neuroectoderm decreased amount sox9.L expression in non-neural ectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.f^3 Anatomical Phenotype increased width of neural plate Expression Phenotype increased amount sox9.L expression in neuroectoderm mislocalised sox9.L expression in neuroectoderm decreased amount sox9.L expression in non-neural ectoderm Xla Wt + dlx3 + NF16 (in situ hybridization) fig.4.g Expression Phenotype decreased amount tubb2b.S expression in neuroectoderm decreased amount tubb2b.S expression in non-neural ectoderm Xla Wt + gata2 + NF16 (in situ hybridization) fig.4.g' Expression Phenotype decreased amount tubb2b.S expression in neuroectoderm decreased amount tubb2b.S expression in non-neural ectoderm Xla Wt + dlx3 MO + NF16 (in situ hybridization) fig.4.g^1 Expression Phenotype decreased amount tubb2b.S expression in neuroectoderm decreased amount tubb2b.S expression in non-neural ectoderm Xla Wt + gata2 MO + NF16 (in situ hybridization) fig.4.g^3 Expression Phenotype decreased amount tubb2b.S expression in neuroectoderm decreased amount tubb2b.S expression in non-neural ectoderm Larger Image Printer Friendly View

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