Xenbase Image ID: 76494

Fig. 3. A highly efficient method of testing promoters and creating transgenic lines using pTransgenesis. (A) Genomic PCR fragments are cloned directly into the p2 position, thus allowing the recombination shown. (B,C) PCR products encoding regions 5′ to nectin-2 and vimentin were generated and tested for transcriptional activity in F0 X. laevis and in F1 X. tropicalis. (D) Transverse sections through the hindbrain and spinal cord of embryos (dorsal side up) stained for endogenous vimentin expression (upper panels), or Venus transgene expression (green) in F0 transgenic X. laevis (middle panels) and F1 transgenic X. tropicalis (lower panels). (E) Transverse sections through the neural plate of stage 18 embryos (dorsal side up) stained for endogenous nectin-2 expression (upper panel), or Venus transgene expression (green) in F0 transgenic X. laevis (middle panel) and F1 transgenic X. tropicalis (lower panel). Nuclei are stained with DAPI (blue) in middle and lower panels in D and E. Image published in: Love NR et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue vim.L vim-a, vim-b, vimentin X. laevis Throughout NF stage 40 hindbrain spinal cord nectin2.L cd112, hveb, LOC100505446, nectin 2, nectin-2, prr2, pvrl2, pvrr2 X. laevis Throughout NF stage 18 neural plate Image source: Published Larger Image Printer Friendly View

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