XB-IMG-76495
Xenbase Image ID: 76495
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Fig. 4. pTransgenesis in various models. (A) Schematic showing plasmid recombinations yielding plasmids compatible with transgenesis in HeLa cells, Xenopus, zebrafish and Drosophila. (B) Images of zebrafish injected with the indicated p1, p2, p3 and p4 elements with or without Tol2 mRNA. Open arrowhead points to activity of the p1 γ-crystallin RFP marker. (C,D) Results from HeLa cell transfections and puromycin (Puro) selection using pTransgenesis-engineered constructions. Red open arrow indicates dying cells. (E) Drosophila melanogaster engineered with the indicated pTransgenesis constructs versus wild type. (F,G) Phase contrast and fluorescence image of pTransgenesis-engineered Drosophila embryo. Open and closed arrowheads indicate gut and cuticle autofluorescence, respectively. (H-J) Confocal images from the indicated Gal4 crosses. (H) srp-Gal4, stage 16 embryo, GFP-labelled hemocytes. (I) eval-Gal4, stage 16 embryo, GFP-labelled CNS. (J) engrailed-Gal4, ventral view of stage15 embryo during dorsal closure process, GFP-labelled engrailed domain segments. Scale bars: 25 μm in C,D; 100 μm in F-J. Image published in: Love NR et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |