Xenbase Image ID: 76551

Figure 4. Recruitment of PG to Stressed Cadherin Adhesions(A and A′) 3D rendered side view of a normal cell injected with Alexa555-dextran (red) and expressing PG-GFP (green) before (A) and after (A′) C-cadFc bead pull. Location of bead, dashed circle.(B′) Cells expressing either PG-GFP (B and B′) or C-cadherin-GFP (C and C′), plated on either FN (B and C) or PLL (B′ and C′) and allowed to form cohesive pairs. Arrowheads indicate plane of cell-cell boundaries.(D) Mesendoderm cells in live tissue expressing PG-GFP (red), mCherry-XCK1(8) (green), and labeled with Alexa647-dextran (gray). Image is a collapsed 2 μm Z-stack of the posterior-lateral region of two adjacent cells in a mesendoderm explant. Outlined region in (D) is shown in independent color channels of plakoglobin-GFP (D′), mCherry-XCK1(8) (D″), and dextran (D″′).(E) C-cadherin and PG were immunoprecipitated from whole embryo extracts and immunoblotted as indicated. α5 integrin immunoprecipitates served as negative controls. All scale bars, 15 μm. Image published in: Weber GF et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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