Xenbase Image ID: 76552

Figure 5. Requirement of PG for Cadherin/Keratin Association(A and B) Single cells labeled with Alexa-dextran, expressing GFP-XCK1(8) (green) and plated on FN. (A) and (A′) show a normal cell (blue dextran), and (B) and (B′) show a PG morphant cell (magenta dextran). C-cadFc bead (circle) bound (A and B), then pulled (A′ and B′).(C and D) Control morphant (blue dextran) (C) and PG morphant (magenta dextran) (D) mesendoderm tissue explants expressing GFP-XCK1(8) (green). See also Movie S8 and Figure S5.(E and F) Control (E) and PG morphant (F) mesendoderm in whole embryos immunostained for XCK1(8) (green) and β-catenin (red). (C) Arrows, cabling along anterior of leading edge cells. Arrowheads, KIF aggregation near cell-cell contacts. All scale bars, 25 μm.(G) Embryos were injected with XCK1(8)-GFP, with or without PG morpholino. (G) Immunoblots of embryo lysates show expression levels of XCK1(8)-GFP and endogenous PG with or without PG morpholino (PG-MO). (H) C-cadherin immunoprecipitates immunoblotted for XCK1(8)-GFP and C-cadherin with or without PG-MO. (I) Quantitation of three independent coimmunoprecipitation experiments shown as mean SEM. Image published in: Weber GF et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page