Xenbase Image ID: 76631

Fig. 4. Nanos1-depleted PGCs exhibit CTD-PSer2 prematurely. (A) Confocal analysis of WT, Nanos1-Ctrl-MO- and Nanos1-MO-injected embryos at stage 11, showing double immunostaining with H5 monoclonal antibody (CTD-PSer2, green) and rabbit anti-Xiwi antibody (germ plasm, red). Merged images are shown at the top, with separate channels beneath. Images were taken from the endoderm core (see the diagram in Fig. 5). Scale bars: 50 μm. (B) PGCs immunopositive for H5 (which is specific for the CTD-PSer2 epitope) in Nanos1-MO (74%) as compared with WT (21%) and Nanos1-Ctrl-MO (16%) embryos. Differences between each group were highly significant by one-way ANOVA. The experiment was repeated three times. Image published in: Lai F et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue polr2a hrpb220, hsrpb1, LOC108703583, polr2, polra, rpbh1, rpiils, rpol2 Xenopus Throughout NF stage 11 germ cell piwil1.L hiwi, LOC108716375, miwi, piwi, xiwi, xiwi1, Xiwi1a, ziwi X. laevis Throughout NF stage 11 germ cell germ plasm Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + nanos1 MO + NF11 (immunohistochemistry) fig.4.a, b Anatomical Phenotype abnormal RNA polymerase II activity Larger Image Printer Friendly View

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