Xenbase Image ID: 76632

Fig. 5. PGCs deficient in Nanos1 activity express endoderm-specific genes. (A,C) RT-PCR analysis of PGCs or endoderm cells isolated from the endoderm core at stage 11 (circled in red, surrounded by endomesoderm in blue) (A) and stage 15 (C). P, primordial germ cells; E, endoderm; WE, whole embryo. Xpat is a germ plasm marker, Xsox17α an endoderm marker, Bix4 an endomesoderm marker and Xbra a mesoderm marker. ODC provides an internal control. The asterisk marks endoderm cell contamination. (B) Confocal analysis of WT, Nanos1-Ctrl-MO- and Nanos1-MO-injected embryos at stage 11. Xsox17α RNA (red) was detected by WISH and PGCs (outlined by dashed line) were identified by Xiwi immunostaining (green). Merged images are shown at the top, with separate channels beneath. Images were taken from the endoderm core. Scale bars: 50 μm. Image published in: Lai F et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 11 endoderm piwil1.L hiwi, LOC108716375, miwi, piwi, xiwi, xiwi1, Xiwi1a, ziwi X. laevis Throughout NF stage 11 germ cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + nanos1 MO + NF11 (immunohistochemistry) fig.5.b Expression Phenotype increased amount sox17a.L expression in primordial germ cell mislocalised sox17a.L expression in primordial germ cell Larger Image Printer Friendly View

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