Xenbase Image ID: 76637

Fig. 2. Deep cells in the non-neural ectoderm actively migrate towards the dorsal side. (A,B) memGFP labeling of deep-layer cells in non-neural ectoderm. Transverse sections of embryos injected with memGFP into a slightly deep area (A). B is a magnified view of the boxed area in A. Arrowheads indicate deep-layer cells. (C,D) Localization of ZO-1 in non-neural ectoderm layers. ZO-1 was localized to the apical junction of superficial cells but not in the deep cells (asterisks). Apical side is upwards and basal towards the bottom. White brackets indicate non-neural ectoderm layer. (E) Trajectories of superficial and deep cell movements in the non-neural ectoderm of a wild-type embryo. Arrow points towards the dorsal side. (F) Cumulative distance from the tracking data of the superficial and deep cells over time. n=3; data are mean±s.e.m. (G) Relative distance between pairs of non-neural superficial cells and the deep cells beneath them during wild-type NTC. Asterisk and bracket indicate cells in the more ventral side. n=9 pairs. Scale bars: 200 μm in A; 100 μm in E; 20 μm in B-D. Image published in: Morita H et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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