XB-IMG-76640
Xenbase Image ID: 76640
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Fig. 5. Inhibition of deep cell movement affects NTC and tension in the superficial cell layer. (A-C) Phenotype of integrin-β1 knockdown in NTC. Transverse sections of late neurula embryos injected with control MO (A), Itgβ1-MO (B) and Itgβ1-MO with rescue mRNA of integrin-β1 (itgβ1-res) (C). The NT of Itgβ1-MO-injected embryos failed to completely close (B, arrow). Arrowheads indicate memGFP-positive cells. (D) Summary of the MO-injected phenotype. Data are mean±s.e.m. *P<0.05, t-test. (E) Trajectories of superficial and deep cell movements in the non-neural ectoderm of Itgβ1-MO-injected embryo. Arrow points towards the dorsal side. (F) Cumulative distance from the tracking data of the Itgβ1-MO-injected cells. n=3; data are mean±s.e.m. (G) Relative distance between pairs of non-neural superficial and deep cells in Itgβ1-MO-injected embryos. n=19 pairs. (H,I) Laser ablation along the AP axis of Itgβ1-MO-injected embryos. Distance (H) and mean angle (I) were measured on the dorsal and ventral sides. n=11, 15, 11 and 18 (from stage 13 to stage 16); data are mean±s.e.m.; n.s., not significant, t-test. (J,K) Relative aspect ratio and angles of the major axis of the non-neural superficial cells in Itgβ1-MO-injected embryos. n=14; data are mean±s.e.m. Itgβ1-res was injected at 250 pg in C and Itgβ1-MO at 10 pmol. Scale bars: 100 μm. Image published in: Morita H et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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