Xenbase Image ID: 76663

Fig. S3. Knockdown of Meis3 and Tsh1 synergizes Meis3 gene expression. (A) Meis3 expression analysis in mid-neurula embryos injected with either the Meis3 MO (23 ng), Tsh1 MO (3 pmol), or both. Although the enhanced expression of Meis3 sustains a relative normal late pattern in either type of knockdown embryo, both deficiencies induce the robustly expanded early expression pattern of Meis3. (B) Overlapping expression of Meis3 and Tsh1 in the hindbrain. Double in situ hybridization to Meis3 (red) and Tsh1 (purple) in mid-neurula embryos. (a) Whole embryo. (b) Higher magnification, bars indicate the overlapping hindbrain expression. (C) Tsh1 represses Meis3 expression cell-autonomously. RT-PCR to Meis3 in mid-neurula stage dispersed AC explants from embryos injected with Tsh1 mRNA (500 pg). Image published in: Elkouby YM et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue meis3.L meis3-a, meis3-b, mrg2, XMeis3 X. laevis Throughout NF stage 18 hindbrain midbrain tshz1.S ny-co-33, sdccag33, teashirt 1a, teashirt1, tsh1, tshz1-a, tshz1-b, XTsh1a X. laevis Throughout NF stage 16 hindbrain meis3.L meis3-a, meis3-b, mrg2, XMeis3 X. laevis Throughout NF stage 16 hindbrain midbrain Image source: Published Larger Image Printer Friendly View

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