XB-IMG-76665
Xenbase Image ID: 76665
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Fig. S5. Meis3 maintenance of proliferative Iro3+ cells at neurula stages is uncoupled from its caudalizing activity at gastrula stages. (A) Iro3 (purple) and Sox3 (red) expression in mid-late neurula embryos co-injected unilaterally with VP16-Meis3 (0.4 ng) and β-galactosidase (β-Gal; 25 pg) mRNAs. β-Gal staining was weakly developed (light blue stain) to allow clear visualization of red and purple probes. Broken line indicates the dorsal mid-line of the embryo, injected β-Gal+ side is to the right. Sox3 and Iro3 expression was co-expanded on the injected side in 6/8 embryos versus 0/16 uninjected control embryos. Note the overlapping Sox3 and Iro3 expression in an ectopic region of β-Gal+ cells (white arrows). (B) sqRT-PCR comparing neuron versus anteroposterior neural marker expression in mid-neurula embryos injected with Meis3-GR (250 pg). Meis3-GR was activated by 1 Dex at either, early gastrula (stage 10), late gastrula (stage 12) or early neurula (stage 14) stages. Activation at early gastrula stages induces Krox20 (hindbrain) and represses Anf1 (forebrain) expression, while n-Tub expression is unaffected. By contrast, while activation at early neurula does not affect Krox20 expression and Anf1 repression is less pronounce, it induces robust Iro3 expression and strongly represses n-Tub expression. Activation at late gastrula seems to represent an intermediate state. This is consistent with the neural plate losing its competence to Wnt/β-catenin caudalizing activity at early neurula stages (Fig. 1B). Image published in: Elkouby YM et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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