Xenbase Image ID: 76760

Figure 8. In situ analysis of selected genes in Xenopus MADM-like (xMADML) morpholino (MO) animals. Embryos injected unilaterally with 18 ng of xMADML-MO at the two-cell stage and showing severely defective eye phenotypes at stage 35 were fixed and assayed for expression of specific genes as indicated below. Each row of panels show staining of one gene. The first column shows the control (non–xMADML-MO injected) side of animals showing the normal in situ expression pattern for each gene. Anterior is to the right and dorsal is up. The second column shows the xMADML-MO–injected side of animals, showing the in situ expression pattern in the knockdown phenotype. Anterior is to the left and dorsal is up. The third column shows a section of the control (uninjected) eye. Distal is up and dorsal is to the left. The fourth column shows a section of the xMADML-MO–injected eye. Distal is up and dorsal is to the right. A–D:Xotx2 staining. E–H:XL-maf staining. I–L: γ6-crystallin staining. M–P:Sox2 staining. Q–T:xSix3 staining. U–X:Sox3 staining. Y–BB:Pax6 staining. The white arrow indicates the eye, the black arrow indicates the lens. nr, neural retina; cns, neural tube. Scale bar = 400 μm in the first two columns, 100 μm in the last two columns.Download figure to PowerPoint Image published in: Elkins MB and Henry JJ (2006) Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 35 and 36 retina retinal neural layer nrl.S l-maf, nrl-maf, rp27, XL-maf, xlmaf X. laevis Throughout NF stage 35 and 36 lens lens fiber cell mass cryga.9.L LOC108702413, LOC447192 X. laevis Throughout NF stage 35 and 36 lens lens fiber cell mass sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 35 and 36 lens retina retinal neural layer retinal inner nuclear layer central nervous system six3.L hpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2 X. laevis Throughout NF stage 35 and 36 retina retinal neural layer sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 35 and 36 lens lens transitional zone central nervous system hindbrain pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 35 and 36 lens retina retinal neural layer lens fiber cell mass Image source: Published Larger Image Printer Friendly View

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