Xenbase Image ID: 76767

Fig. S2. A p3 library of fluorescent proteins. (A) Fifteen fluorescent proteins with the approximate peak emissions indicated were incorporated into the p3 coding sequence position of pTransgenesis. Note: Kaede* and Kikume* possess red-shifted emissions following photoactivation. (B) The p3 fluorescent proteins were linked downstream of the p2 CMV promoter and with a p1 γ-crystallin selection marker. (C) Resulting constructs were tested for functionality via REMI. Note: fluorescence was captured using CFP, GFP or RFP filters and then pseudocoloured to represent each protein's peak emission more accurately. The Kaede protein has undergone a red-green colour shift after exposure to 405 nm light. Image published in: Love NR et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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