Xenbase Image ID: 76768

Fig. S3. Establishment of X. laevis transgenic lines amenable to Gal4-UAS binary transgene expression. (A,B) Schematics showing the construction of Gal4 and UAS constructs using pTransgenesis plasmids. (C-E) Merged brightfield and fluorescent exposures of single transgenic embryos containing the indicated Gal4 or UAS drivers. (F-H) Merged brightfield and fluorescent exposures of double transgenics. The arrowheads in G and H show a morphological defect in the tail in severe and less severe phenotypes. (I) Schematic of F0 founder crossings. (J) Brightfield images showing resulting F1 transgenic tadpoles, with double transgenics showing germline transmission of tail and body length defect. (K) Merged fluorescence of GFP and RFP channels for the tadpoles shown in I. Image published in: Love NR et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page