Xenbase Image ID: 76789

FIGURE 3. Dorsalization is pronounced in tadpoles overexpressing XSMOC-1. A, stage 26 Xenopus embryos injected bilaterally at the two-cell stage with 300 pg of GFP (A, C, and E) or XSMOC-1 mRNAs (B, D, and F). XSMOC-1-injected embryos were dorsalized, with exaggerated dorsal/anterior structures, particularly cement glands. The XSMOC-1 overexpression phenotypes shown in B were typical for this stage and were observed in 95% of the embryos in five independent experiments (n=218). C and D, histological 3-mm plastic sections (modified Von Gieson stain) showing hypertrophic cement gland cells in XSMOC-1-overexpressing embryos (D). E and F, 7-mm paraffin sections (Feulgen, light green, orange G method) showing enlargement of the neural tube and disorganized somites in XSMOC-1 over-expressing embryos (F). "This research was originally published THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL.284(28) pp.18994-19005, July 10, 2009. Copyright: The American Society for Biochemistry and Molecular Biology." Image published in: Thomas JT et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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