XB-IMG-76792
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FIGURE 6. Complete loss of XSMOC-1 function leads to developmental arrest prior to neurulation. A-F, embryos injected bilaterally at the two-cell stage with 6 ng of 5-base mismatch control (A) or antisense (D-F) XSMOC-1 MO. Control MO-injected embryos developed normally (the position of the neural tube is indicated in C by an arrow), whereas antisense MO-injected embryos appeared normal up to the end of gastrulation (stage 12) but arrested prior to neurulation. The XSMOC-1 MO phenotypes shown in F were typical for this stage and were observed in 95% of the embryos in eight independent experiments (n = 326). G-I, RT-PCR analyses of markers expressed by control and antisense-XSMOC-1 MO-injected embryos at stage 10.5 (G),
12 (H), and 15 (I). Marker expression appeared normal up to stage 12 (G and H), but markers normally expressed after gastrulation were diminished (I). -RT, without reverse transcriptase.
"This research was originally published THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL.284(28) pp.18994-19005, July 10, 2009. Copyright: The American Society for Biochemistry and Molecular Biology." Image published in: Thomas JT et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |