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Figure 8. Noggin and Dkk-1 regulate the expression of newly identified genes. 4-cell embryos were ventrally injected with the noggin and/or dkk-1 concentrations described in Figure 1, and tested by in situ hybridization for patterns of ectopic induction or repression. In each case the observed in situ patterns confirm the microarray patterns (A-D) LRIG2 shows ectopic ventral expression in both the Noggin and the Noggin+Dkk-1 overexpressing embryos, but not in the Dkk-1 embryos. (E-H) ARHGEF3 shows ectopic ventral expression only in the Noggin+Dkk-1 overexpressing embryos. (I-L) HES6 shows ectopic ventral repression in the Noggin+Dkk-1 and the Dkk-1 overexpressing embryos. (M-P) Frzb3 expression is ectopically repressed by Noggin or Noggin+Dkk-1 overexpression. (Q-T) Xl.3529.1.A1 expression is ectopically repressed by Noggin+Dkk-1 and Dkk-1 overexpression. (U-X) Gadd45g is ectopically induced only in the Noggin+Dkk-1 condition. All embryos are between stages 10.5 and 11 and are shown in vegetal view with dorsal side facing up. Image published in: Hufton AL et al. (2006) Copyright © 2006 Hufton et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue lrig2.L X. laevis Throughout NF stage 10.5 to NF stage 11 involuted dorsal mesoderm upper blastopore lip dorsal marginal zone aplnr.L agtrl1, angio1, apj, apjr, aplnr-a, aplnr-b, hg11, msr, X-msr, Xangio1, Xmsr X. laevis Throughout NF stage 10.5 to NF stage 11 involuted dorsal mesoderm marginal zone hes6.1.L Xhes-6, XHes6 X. laevis Throughout NF stage 10.5 to NF stage 11 ventral marginal zone ventro-lateral marginal zone dorso-lateral marginal zone szl.L frzb3, secreted frizzled, sizzled, xszl X. laevis Throughout NF stage 10.5 to NF stage 11 ventral marginal zone gadd45g.L XGadd45-gamma, xgadd45g X. laevis Throughout NF stage 10.5 to NF stage 11 involuted dorsal mesoderm upper blastopore lip Image source: Published Larger Image Printer Friendly View

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